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恶臭假单胞菌KT转化左旋葡聚糖和纤维二糖-外文文献论文翻译

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发布于 2017-1-28 14:27:15 | 显示全部楼层 |阅读模式
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外文标题: Conversion of levoglucosan and cellobiosan by Pseudomonas putida KT2440
外文摘要: Pyrolysis offers a straightforward approach for the deconstruction of plant cell wall polymers into bio-oil. Recently, there has been substantial interest in bio-oil fractionation and subsequent use of biological approaches to selectively upgrade some of the resulting fractions. A fraction of particular interest for biological upgrading consists of polysaccharide-derived substrates including sugars and sugar dehy- dration products such as levoglucosan and cellobiosan, which are two of the most abundant pyrolysis products of cellulose. Levoglucosan can be converted to glucose-6-phosphate through the use of a le- voglucosan kinase (LGK), but to date, the mechanism for cellobiosan utilization has not been demon- strated. Here, we engineer the microbe Pseudomonas putida KT2440 to use levoglucosan as a sole carbon
and energy source through LGK integration. Moreover, we demonstrate that cellobiosan can be en- zymatically converted to levoglucosan and glucose with β-glucosidase enzymes from both Glycoside Hydrolase Family 1 and Family 3. β-glucosidases are commonly used in both natural and industrial cellulase cocktails to convert cellobiose to glucose to relieve cellulase product inhibition and to facilitate microbial uptake of glucose. Using an exogenous β-glucosidase, we demonstrate that the engineered strain of P. putida can grow on levoglucosan up to 60 g/L and can also utilize cellobiosan. Overall, this
study elucidates the biological pathway to co-utilize levoglucosan and cellobiosan, which will be a key transformation for the biological upgrading of pyrolysis-derived substrates.
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外文年份: 2016
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译文标题: 恶臭假单胞菌KT转化左旋葡聚糖和聚纤维二糖
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译文摘要: 高温分解可以轻易解构植物细胞壁聚合物并使其转化为生物原油。近年,人们对生物油分馏及后续进一步提取分馏物有极大的兴趣。部分对生物升级感兴趣的人聚焦多糖衍生的底物,包括糖类和脱水糖产物如左旋葡聚糖和聚纤维二糖,这种两种糖是纤维素经过高温分解后含量最大的产物。通过左旋葡聚糖激酶,左旋葡聚糖可转化为葡萄糖-6-磷酸。但到目前为止,还没发现利用聚纤维二糖的方法。笔者改造了微生物恶臭假单胞菌KT,通过左旋葡聚糖激酶的融合,将左旋葡聚糖作为碳和能量的唯一来源。此外,本文试验表明利用糖苷水解酶家族1和糖苷水解酶家族3的β-葡糖苷酶,聚纤维二糖可以酶法转换为左旋葡聚糖和葡萄糖。β-葡糖苷酶通常用于自然或工业的纤维素酶混合物中,将纤维二糖转化为葡萄糖以缓解纤维素酶产物抑制和促进微生物摄取葡萄糖。本文试验表明使用外源性β-葡萄糖苷酶,恶臭假单胞菌的工程菌株可在左旋葡聚糖上生长至浓度为60 g/L并仍利用聚纤维二糖。总之,本文研究阐明了共同利用左旋葡聚糖和纤维二糖的生物学途径,这将是改变热解衍生底物的生物升级的关键因素。
译文格式: WORD
中关键词: 关键词: 恶臭假单胞菌KT 左旋葡聚糖激酶 糖苷水解酶 聚纤维二糖 高温分解
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