请选择 进入手机版 | 继续访问电脑版

休息抑制了胰腺内分泌分化程序的一个子集

[复制链接]
发布于 2017-8-14 16:58:30 | 显示全部楼层 |阅读模式
外文翻译
推荐等级:  
外文概要: -
外文标题: REST represses a subset of the pancreatic endocrine differentiation program
外文摘要: To contribute to devise successful beta-cell differentiation strategies for the cure of Type 1 diabetes we sought to uncover barriers that restrict endocrine fate acquisition by studying the role of the tran- scriptional repressor REST in the developing pancreas. Rest expression is prevented in neurons and in endocrine cells, which is necessary for their normal function. During development, REST represses a subset of genes in the neuronal differentiation program and Rest is down-regulated as neurons differ- entiate. Here, we investigate the role of REST in the differentiation of pancreatic endocrine cells, which are molecularly close to neurons. We show that Rest is widely expressed in pancreas progenitors and that it is down-regulated in differentiated endocrine cells. Sustained expression of REST in Pdx1 t progenitors impairs the differentiation of endocrine-committed Neurog3 t progenitors, decreases beta and alpha cell mass by E18.5, and triggers diabetes in adulthood. Conditional inactivation of Rest in Pdx1 t progenitors is not sufficient to trigger endocrine differentiation but up-regulates a subset of differentiation genes. Our results show that the transcriptional repressor REST is active in pancreas progenitors where it gates the activation of part of the beta cell differentiation program.
英关键词: NRSF Beta cells Diabetes Islets Repressor Pancreas
正文摘录: Type 1 diabetes (T1D) is caused by an absolute deficiency of insulin secretion due to autoimmune destruction of insulin-se- creting beta cells and can be treated by islet cell replacement therapy. However, the lack of high quality donor cells has prompted the search for alternative sources of beta cells to try generating an unlimited supply of beta cells for transplantation. There are promising findings demonstrating in vivo transdiffer- entiation of acinar, alpha or delta cells into beta cells (Chera et al., 2014; Collombat et al., 2009; Zhou et al., 2008) or differentiation of human embryonic stem cells (ESC) to islet cells (Kroon et al., 2008; Pagliuca et al., 2014; Rezania et al., 2014). However, the differ- entiation of these cells into beta cells is often partial, a problem which can potentially be solved by better understanding how beta cells differentiate during pancreas embryogenesis.
外文格式: 全英文PDF
外文结构: -
外文单词: 10564
外文字符: 56936
外文出处: Developmental Biology 405 (2015) 316–327
外文年份: 2015
外文附图:
译文标题: 休息抑制了胰腺内分泌分化程序的一个子集
译文概要: -
译文摘要: -
译文格式: WORD
中关键词: -
译文字数: -
译文作者: -
外文性质: 定制外文 
外文详情: -

REST-represses-a-subset-of-the-pancreatic-endocrine-dif_2015_Developmental-B.pdf

 
若上述预览未显示,请刷新页面。提供专业人工翻译服务,可联系客服,QQ:1799162066
 

REST-represses-a-subset-of-the-pancreatic-endocrine-dif_2015_Developmental-B.pdf

7.2 MB

检索服务费: 10 元  [购买]

回复

使用道具 举报

展开
快速回复 返回顶部 返回列表